imagequant analysis Search Results


phorphorimager analysis program imagequant  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc phorphorimager analysis program imagequant
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Phorphorimager Analysis Program Imagequant, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagequant image analysis system storm optical scanner  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc imagequant image analysis system storm optical scanner
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Imagequant Image Analysis System Storm Optical Scanner, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagequant image analysis system storm optical scanner - by Bioz Stars, 2026-04
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1d gel analysis imagequant tl software  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc 1d gel analysis imagequant tl software
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
1d Gel Analysis Imagequant Tl Software, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1d gel analysis imagequant tl software/product/Molecular Dynamics Inc
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1d gel analysis imagequant tl software - by Bioz Stars, 2026-04
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computing phosphorescence imager phosphorimager with imagequant software analysis  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc computing phosphorescence imager phosphorimager with imagequant software analysis
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Computing Phosphorescence Imager Phosphorimager With Imagequant Software Analysis, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel analysis program imagequant  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc gel analysis program imagequant
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Gel Analysis Program Imagequant, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gel analysis program imagequant/product/Molecular Dynamics Inc
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gel analysis program imagequant - by Bioz Stars, 2026-04
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imagequant fragment analysis software  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc imagequant fragment analysis software
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Imagequant Fragment Analysis Software, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imagequant fragment analysis software/product/Molecular Dynamics Inc
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imagequant fragment analysis software - by Bioz Stars, 2026-04
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imagequant software coupled to a storm 860  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc imagequant software coupled to a storm 860
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Imagequant Software Coupled To A Storm 860, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagequant software coupled to a storm 860 - by Bioz Stars, 2026-04
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quantitative phosphoimage analysis imagequant  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc quantitative phosphoimage analysis imagequant
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Quantitative Phosphoimage Analysis Imagequant, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative phosphoimage analysis imagequant - by Bioz Stars, 2026-04
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chemiluminescence imaging analysis system imagequant las4000mini  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc chemiluminescence imaging analysis system imagequant las4000mini
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Chemiluminescence Imaging Analysis System Imagequant Las4000mini, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagequant analysis program  (FUJIFILM)
90
FUJIFILM imagequant analysis program
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Imagequant Analysis Program, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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array analysis component of imagequant tl software  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc array analysis component of imagequant tl software
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Array Analysis Component Of Imagequant Tl Software, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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analysis program imagequant 3.3  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc analysis program imagequant 3.3
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Analysis Program Imagequant 3.3, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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analysis program imagequant 3.3 - by Bioz Stars, 2026-04
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Image Search Results


Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program ImageQuant. Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.

Journal:

Article Title: Evidence for κ - and μ -Opioid Receptor Expression in C6 Glioma Cells

doi:

Figure Lengend Snippet: Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program ImageQuant. Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.

Article Snippet: Band densities were quantified with the phorphorimager analysis program ImageQuant by Molecular Dynamics.

Techniques: Expressing, Amplification, Electrophoresis, Southern Blot, Sequencing, Comparison, Control